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a , Representative anti-His tag-APC versus SSC-A dot plots for PQN4 displaying CsgF fused with protein of interest (POI), gated region based on negative control of CsgF only strain and positive control of CsgF-His tag strain. b , Representative <t>anti-FLAG</t> tag-PE versus SSC-A dot plots for PQN4 displaying FLAG tag or not. c , Representative Spy Catcher-FITC versus SSC-A dot plots for PQN4 displaying Spy tag or not.
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a , Representative anti-His tag-APC versus SSC-A dot plots for PQN4 displaying CsgF fused with protein of interest (POI), gated region based on negative control of CsgF only strain and positive control of CsgF-His tag strain. b , Representative anti-FLAG tag-PE versus SSC-A dot plots for PQN4 displaying FLAG tag or not. c , Representative Spy Catcher-FITC versus SSC-A dot plots for PQN4 displaying Spy tag or not.

Journal: bioRxiv

Article Title: Programming cellular condensates for living materials using an intrinsically disordered protein display platform (iDP 2 )

doi: 10.1101/2025.03.02.640994

Figure Lengend Snippet: a , Representative anti-His tag-APC versus SSC-A dot plots for PQN4 displaying CsgF fused with protein of interest (POI), gated region based on negative control of CsgF only strain and positive control of CsgF-His tag strain. b , Representative anti-FLAG tag-PE versus SSC-A dot plots for PQN4 displaying FLAG tag or not. c , Representative Spy Catcher-FITC versus SSC-A dot plots for PQN4 displaying Spy tag or not.

Article Snippet: After washing, 200 µL Alexa488 anti-His Tag Antibody (Thermo Fisher, #MA1-21315-A488, 1:100 dilution) for His tag staining, Alexa594 anti-FLAG (DYKDDDDK) Tag Antibody (Novus Biologicals™ 1:100 dilution) for FLAG tag staining, or both was added for overnight incubation at 4 °C.

Techniques: Negative Control, Positive Control, FLAG-tag